Terminator

Part:BBa_K2598054:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-10-03)


Strong synthetic terminator L3S3P11


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 40


Design Notes

Source

This is obtained by Chen et al and is provided by Christopher A Voigt.

References

Chen, Y. J. et al. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods 10, 659-664, (2013).

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)